Prevalence and molecular diagnosis of Gongylonema pulchrum in cattle and sheep in the Samsun region

This study was carried out between November-2017 and June-2019 to determine the distribution molecular characterization of Gongylonema pulchrum in cattle sheep slaughtered local abattoirs slaughtering areas during feast sacrifice. In this study, esophagi collected from 380 (358 young 22 mature) 848 (816 32 old) were examined. Esophagus taken after brought laboratory plastic bag. laboratory, all cut open with scissors materials examined using a loupe under light source. Parasites eye forceps placed 70% ethyl alcohol. Following primary morphological examination, parasites into three parts, anterior posterior parts used for morphologic identification mid part identification. Bp BpCoxI-F1 BpCoxI-R1 primers Only two cows (older than four years) infected infection rate 0.53%. A total 27 identified, 18 male 9 female. not found sheep. All samples identified as G. examinations confirmation positive bands detected at 400bp.